GENETIC APPLICATIONS


COMPARATIVE GENOMIC HYBRIDIZATION (CGH)

  • Achieving a desired pregnancy and giving birth to a healthy baby depends on whether the embryo has a healthy chromosome structure or not. If the chromosome structure is not healthy, mostly the body cannot realized pregnancy or the pregnancy results in a miscarriage. If pregnancy cannot be achieved after Recurrent IVF method or the pregnancy resulted in miscarriage and moreover, if normal pregnancies result in miscarriage, the reason must be investigated. In such conditions, it is necessary to investigate whether there is genetic defect or not.

    CGH is applied successfully to females who are over 38, who had embryo transfer although any problem is not detected and who had at least 2 unsuccessful IVF, who had at least 1 normal embryo detected with PGD and transferred before but are not pregnant and who had chromosomal defect due to Recurrent miscarriages. Ova of the future mothers who come to the center for IVF are collected after medication.

    They are grown and developed by being inseminated with the male sperm with microinjection (ICSI) operation in laboratory environment. In the 3rd day of the growth, a cell is taken from embryos with 6, 7 and 8 cells by opening a hole in the membrane of the embryo by means of laser under microscope. This operation which is carried out by experienced people and advanced equipment do not harm the embryo. Rest of the embryos split up and continue their development.

    One cell taken from each embryo is examined with Microarray based Comparative Genomic Hybridization (CGH) method.

    Unlike previously applied PGD (pre-implantation genetic diagnosis), all chromosomes are examined with this method. According to the results obtained 24 hours later, healthy embryo is transferred to the mother’s womb. With IVF method, pregnancy rates, which are 45-55% are increased to 70-75%. While live birth rates increase with this method, the rates of miscarriage has reduced. With CGH method, pregnancy rates reach up to 30-40% in patients over 38. With patients who had 4-5 times Recurrent miscarriages, live birth rates increased by 60-70% with CGH method.